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mitochondrial isolation buffer  (Beyotime)


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    Structured Review

    Beyotime mitochondrial isolation buffer
    Mitochondrial Isolation Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondrial+isolation+buffer/Mitochondria+Isolation+Reagent+B/pm41184641-322-8-12
    Average 99 stars, based on 1119 article reviews
    mitochondrial isolation buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: The aldehyde dehydrogenase ALDH1B1 exerts antiviral effects through the aggregation of the adaptor MAVS.
    Article Snippet: Type I interferons (IFNs) are produced by almost all cell types and play a vital role in host defense against viral infection.. Infection with an RNA virus activates receptors such as RIGI, resulting in the recruitment of the adaptor protein MAVS to the RIGI–like receptor (RLR) signalosome and the formation of prionlike functional aggregates of MAVS, which leads to IFNβ production.. Here, we identified the aldehyde dehydrogenase 1B1 (ALDH1B1) as a previously uncharacterized IFNstimulated gene (ISG) product with critical roles in the antiviral response.

    Homogenization:

    Article Title: The aldehyde dehydrogenase ALDH1B1 exerts antiviral effects through the aggregation of the adaptor MAVS.
    Article Snippet: Type I interferons (IFNs) are produced by almost all cell types and play a vital role in host defense against viral infection.. Infection with an RNA virus activates receptors such as RIGI, resulting in the recruitment of the adaptor protein MAVS to the RIGI–like receptor (RLR) signalosome and the formation of prionlike functional aggregates of MAVS, which leads to IFNβ production.. Here, we identified the aldehyde dehydrogenase 1B1 (ALDH1B1) as a previously uncharacterized IFNstimulated gene (ISG) product with critical roles in the antiviral response.

    Isolation:

    Article Title: The aldehyde dehydrogenase ALDH1B1 exerts antiviral effects through the aggregation of the adaptor MAVS.
    Article Snippet: Type I interferons (IFNs) are produced by almost all cell types and play a vital role in host defense against viral infection.. Infection with an RNA virus activates receptors such as RIGI, resulting in the recruitment of the adaptor protein MAVS to the RIGI–like receptor (RLR) signalosome and the formation of prionlike functional aggregates of MAVS, which leads to IFNβ production.. Here, we identified the aldehyde dehydrogenase 1B1 (ALDH1B1) as a previously uncharacterized IFNstimulated gene (ISG) product with critical roles in the antiviral response.

    Article Title: Downhill running induced mitophagy in rat soleus muscle via the AMPK pathway.
    Article Snippet: 1 School of Sports Medicine and Health, Chengdu Sport University, Chengdu, China 2 School of Sport Science, Beijing Sport University, Beijing, China 3 School of Physical Education, Liaoning Normal University, Dalian, China 4 Department of Physical Education, Central South University, Changsha, China 5 College of Physical Education and Health, North Campus, Wenzhou University, Chashan University Town, Wenzhou 325035, P. R. China 6 Key Lab of Health-Related Physical Fitness of Wenzhou, Wenzhou, China Abstract Eccentric exercise is known to induce more pronounced muscle damage associated with delayed-onset muscle soreness than concentric exercise.. This study aimed to investigate whether AMP-activated protein kinase (AMPK) pathway participates in control of mitophagy in rat skeletal muscle in response to downhill running.. Eighty-eight male Sprague-Dawley rats were exercised on a treadmill tilted at 16° decline at 16 m·min− 1 for 90 min, with the soleus muscle sampled at 0 h, 12 h, 24 h, 48 h and 72 h after exercise.

    Article Title: Knockout of the sulfide: quinone oxidoreductase SQR reduces growth of HCT116 tumor xenograft.
    Article Snippet: Colorectal cancer (CRC) exhibits significant diversity and heterogeneity, posing a requirement for novel therapeutic targets.. Polysulfides are associated with CRC progression and immune evasion, but the underlying mechanisms are not fully understood.. Sulfide: quinone oxidoreductase (SQR), a mitochondrial flavoprotein, catalyzes hydrogen sulfide (H2S) oxidation and polysulfides production.

    Article Title: IDR-driven TOLLIP condensates antagonize the innate antiviral immunity by promoting the deSUMOylation of MAVS.
    Article Snippet: .. Cells were washed by pre-chilled PBS two times, then harvested in Mitochondrial isolation buffer (Beyotime Biotechnology) followed by dounce-homogenization. ..

    Article Title: Mitochondria-transliterated ALDH1L1 functions as a feedback regulator of redox homeostasis in cancer cells to enhance the resistance to pro-oxidative therapy.
    Article Snippet: To prevent cell death induced by elevated oxidative stress, cancer cells activate a series of antioxidant defense mechanisms to mitigate cytotoxicity, thereby enhancing the resistance to pro-oxidative therapy.. However, the underlying antioxidant mechanisms in cancer cells remain inadequately understood.. Through co-immunoprecipitation followed by quantitative mass spectrometry analysis, we for the first time identified that cytoplasmic ALDH1L1 translocates into mitochondria and co-localizes with mitochondrial transcription factor TFAM in cancer cells in a ROS-dependent feedback manner.

    Article Title: Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway.
    Article Snippet: .. These cells were then resuspended in a mitochondrial isolation buffer (C3601, Beyotime Biotechnology) for 15 min, the cell homogenate was centrifuged at 600 ×g for 10 min at 4 ◦C, resulting in a mitochondrial suspension. ..

    Article Title: Knockout of the sulfide: quinone oxidoreductase SQR reduces growth of HCT116 tumor xenograft
    Article Snippet: .. A total of 2 × 10 6 cells were collected and resuspended in 1 ml of ice-cold mitochondrial isolation buffer (Beyotime Biotechnology, C3601). ..

    Article Title: MIRO ‐1 interacts with VDAC ‐1 to regulate mitochondrial membrane potential in Caenorhabditis elegans
    Article Snippet: .. The isolated mitochondria were resuspended in a mitochondrial isolation buffer containing diluted JC‐1 staining dye (Beyotime, C2005, China) for 15 min. ..

    Suspension:

    Article Title: Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway.
    Article Snippet: .. These cells were then resuspended in a mitochondrial isolation buffer (C3601, Beyotime Biotechnology) for 15 min, the cell homogenate was centrifuged at 600 ×g for 10 min at 4 ◦C, resulting in a mitochondrial suspension. ..

    Staining:

    Article Title: MIRO ‐1 interacts with VDAC ‐1 to regulate mitochondrial membrane potential in Caenorhabditis elegans
    Article Snippet: .. The isolated mitochondria were resuspended in a mitochondrial isolation buffer containing diluted JC‐1 staining dye (Beyotime, C2005, China) for 15 min. ..



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